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IPTG 異丙基-β-D-硫代半乳糖苷 CAS:367-93-1

產品名稱: IPTG 異丙基-β-D-硫代半乳糖苷 CAS:367-93-1(植物來源non-animal origin 美國藥典U P32標準級)
地區: 浙江省 寧波市 鎮海區
付款方式: TT
生產率: 1000KGS/MONTH
包裝: 25公斤裝
交貨期限: 7天
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詳細信息

外觀性狀:白色粉末 含量:99.8(%)

產品摘要

白色結晶粉末;易溶於水、,可溶於、不溶於。是β-半乳糖苷酶和β-半乳糖透酶的誘導劑,不被β-半乳糖苷水解,是硫代半乳糖轉酰酶的底物

產品描述

產品:IPTG 異丙基-β-D-硫代半乳糖苷 CAS:367-93-1


IPTG 異丙基-β-D-硫代半乳糖苷 CAS:367-93-1(植物來源non-animal origin 美國藥典U P32標準級

性狀描述

白色結晶粉末;易溶於水、,可溶於、,不溶於。是β-半乳糖苷酶和β-半乳糖透酶的誘導劑,不被β-半乳糖苷水解,是硫代半乳糖轉酰酶的底物。

熔點:114~121℃

分子式:C9H18O5S

用途說明

常用分子生物學試劑,常用於藍白斑篩選及IPTG誘導的細*內的蛋白表達等。

乳糖類似物 IPTG的主要功能是解除阻遏物對 Lac或 Tac啟動子,誘導重組蛋白 bFGF的產生,采用搖瓶實驗測定了 IPTG對E.coliJM103的生長的影響。

IPTG對不含質粒的E.coliJM103的*大生長速率和*終細胞密度無明顯影響,而對於含質粒的E.coliJM
103-pUC18-bFGF的*大生長速率和*終細胞密度不論是否加入 IPTG,其結果均比不含質粒的JM103低,說明含外源基因的細胞不論外源基因表達與否,都能影響宿主細胞的生長,只不過外源基因在表達後,其對宿主細胞的生長影響表現得更加明顯。

用IPTG誘導表達有活性的色氨酸酶,IPTG濃度對色氨酸酶的活性有較大的影響,而且低溫有利於活性表達,但是,太低的溫度會影響菌體的生長速度,延長發酵周期。

包裝規格

1G, 5G, 25G, 100G, 1KG, 25KG

IPTG  Isopropyl-beta-D-thiogalactoside CAS:367-93-1

 

Character description

White crystalline powder; easy to dissolve in water, methanol, ethanol, soluble in acetone, chloroform, insoluble in ether. It is the inducer of beta galactosidase and beta galactotransaminase, which is not hydrolyzed by beta galactoside and is the substrate of thiosulfate galactoylase.

Melting point: 114~121 degrees centigrade

Molecular formula: C9H18O5S

Use description

The commonly used molecular biology reagents, commonly used in blue white screening and IPTG induced protein expression in bacteria.

The main function of lactose analogue IPTG is to release the repressor from Lac or Tac promoter and induce the production of recombinant protein bFGF. The effect of IPTG on E.coliJM103 growth was determined by shake flask test.

IPTG had no significant effect on the maximum growth rate and the final cell density of the plasmid free E.coliJM103, but for the plasmid containing E.coliJM

The maximum growth rate of 103-pUC18-bFGF and the final cell density regardless of whether to join the IPTG, the results are less than the plasmid containing JM103 is low, indicating whether the exogenous gene expressing cells containing exogenous gene or not, can affect the host cell growth, but in the expression of exogenous gene, its effect on the growth of the host cell is more obviously.

The activity of tryptophanase was induced by IPTG. The concentration of IPTG had great influence on the activity of tryptophanase, and low temperature was beneficial to the expression of activity. However, too low temperature would affect the growth rate of bacteria and prolong the fermentation period.

Packaging specification

1G, 5G, 25G, 100G, 1KG, 25KG

Storage conditions

Cold storage at 2-8 centigrade.

Test report:

1.PH value: 5.86

2. clarification degree: colorless clarification

3. absorbance: A330nm:0.073; A400nm:0.038

4. rotation: -29.6 degrees

5. water: 0.8%

6. melting point: 118.7-123.7

7. Purity: 99.5%

Use: IPTG is beta - use. Nowadays, IPTG has been used in more and more fields. It plays a huge role in the fields of production, pharmaceuticals, biotechnology and so on, bringing greater benefits to enterprises, improving product quality and shortening product response time.



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